Reticulocyte Dye Kwa Sysmex Hematology Analyzers

Dye ya Reticulocyte ya Sysmex Hematology Analyzers imapereka utoto wa fulorosenti wa nucleic acid pamapulatifomu a semiconductor laser flow cytometry. The reagent yamadzimadzi imalowa m'maselo amagazi, ma reticulocyte, ndi mapulateleti kuti amangirire mosankha ndi RNA ndi DNA, ndikupanga ma fluorescence am'mbali ndikubalalitsa kuwala pamakina odziwika. Yapangidwa kuti ilumikizidwe mwachindunji ndi makina opangira ma fluidic, yankho lake limasunga mphamvu ya fluorescence yosasunthika, phokoso lochepa kumbuyo, ndi zambiri zomwe zingapangidwenso-kuti{3}}zigwire bwino ntchito m'ma labotale achipatala.

Makina opangira utoto wa hematology ndi zolembera za fluorescent zomwe zimapangidwira kuti ziziyenda-cytometry{{1}kutengera nsanja zowunikira ma cell. Mosiyana ndi madontho opaka magazi opaka pamanja omwe amagwiritsidwa ntchito mu maikolosikopu owala, utoto wamadzimadziwu umalowa m'maselo omwe amatsata kudzera mu membrane permeabilization kuti alembe ma intracellular nucleic acid. Pansi pa chisangalalo cha semiconductor laser mkati mwa cell optical flow cell, zolembedwa zama cell zimatulutsa ma fluorescence am'mbali ndi ma siginecha obalalitsa kuwala, kulola ma optics a system kuti apange ma scattergram awiri enieni ndi magawo awiri.

Pakuwunika kwa hematology ya optical, utoto wa fulorosenti umathandizira kusankhana kwa ma cell poyesa kuchuluka kwa ma intracellular nucleic acid:

Kusiyana kwa Maselo Oyera a Magazi:Amalekanitsa ma lymphocytes, monocytes, neutrophils, eosinophils, ndi basophils kutengera RNA / DNA ratios ndi cytoplasmic structure.

Chizindikiritso cha Ma cell Atypical & Immature:Imazindikiritsa maselo ophulika ndi ma granulocyte osakhwima kudzera mumphamvu yokwezeka ya fluorescence.

Kuwerengera kwa Reticulocyte:Imayesa otsalira a ribosomal RNA m'maselo ofiira achichepere kuti awone momwe m'mafupa amagwirira ntchito erythropoietic.

Kuwerengera kwa Platelet Kuwala:Amasungunula mapulateleti akuluakulu ndikusiyanitsa tizidutswa ta maselo ofiira a m'magazi ndi mapulateleti pogwiritsa ntchito chandamale cha nucleic acid fluorescence geting.

Utoto wa fulorosenti umagwira ntchito pamakina apadera odzipangira okha:

Channel ya WNR:Imawerengera maselo oyera amwazi ndikupatula maselo ofiira a nucleated kudzera mumitundu yosiyanasiyana ya nucleic acid.

WDF Channel:Imasiyanitsa okhwima a leukocyte subpopulations ndipo amazindikira ma granulocyte osakhwima pogwiritsa ntchito mbali fulorosisi ndi mbali zobalalitsa magawo.

WPC Channel:Zowonetsera zama cell a progenitor ndi ma lymphocyte achilendo kutengera kachulukidwe ka nyukiliya komanso kusiyanasiyana kwa membrane permeability.

RET Channel:Imayezera tizigawo ting'onoting'ono ta reticulocyte ndi mawonekedwe a maselo ofiira amagazi kudzera mumphamvu ya RNA fluorescence.

PLT Channel:Amalemba mapulateleti a nucleic acid kuti athetse kusokoneza kwa maselo ofiira a m'magazi a microcytic panthawi yowerengera.

Amapangidwa kuti azilumikizana mwachindunji ndi nsanja za hematology zogwiritsa ntchito semiconductor laser flow cytometry:

Mndandanda wa XN:XN-1000, XN-2000, XN-3000, XN-9000, XN-L Series (XN-350, XN-450, XN-550)

Mndandanda wa XS:XS-500i, XS-800i, XS-1000i

Mndandanda wa XT:XT-1800i, XT-2000i, XT-4000i

XE Series:XE-2100, XE-5000

WNR Channel Staining (STAC-DYE-SYSWNR):Imalowetsa ma membrane am'maselo kuti alole kulowa mwachangu kwa utoto wa nyukiliya, kulekanitsa ma nuclei a NRBC kuchokera ku maselo oyera amagazi kuti atsimikizire bwino za NRBC% ndi WBC count.

WDF Channel Staining (STAC-DYE-SYSWDF):Madontho a intracellular nucleic acids a leukocytes kuti apange masango a scattergram a neutrophils, ma lymphocytes, monocytes, ndi eosinophils.

WPC Channel Staining (STAC-DYE-SYSWPC):Kusankha kumadetsa ma cell ophulika ndi ma lymphocyte osadziwika kutengera kapangidwe ka lipid ndi kachulukidwe ka nyukiliya pama cell achiwiri.

RET Channel Staining (STAC-DYE-SYSRET):Amamangirira ku RNA yotsalira m'maselo ofiira ofiira, zomwe zimathandiza kuwerengera kuchuluka kwa reticulocyte peresenti, kuwerengera kwathunthu, ndi kachigawo kakang'ono ka reticulocyte.

PLT Channel Staining (STAC-DYE-SYSPLT):Amalemba ma platelet nucleic acid kuti ateteze kusokoneza kwa maselo ofiira a m'magazi aang'ono ang'onoang'ono-pamiyeso yocheperako- yoyezera ma platelet.

Masanjidwe Pakuyika:

• Makaseti a cartridge: 2 × 12 mL
• Njira yothetsera mabotolo: 1 × 50 mL, 1 × 100 mL, 1 × 500 mL

Kutentha Kosungirako:2 digiri mpaka 30 digiri (Tetezani ku kuwala kwachindunji ndi kuzizira)
Moyo Wama Shelufu Wosatsegulidwa:18 miyezi
Pa-Kukhazikika Pabwalo:60 masiku yozungulira zasayansi zinthu

Mayankho a Hematology Diluent:Mayankho a isotonic buffer omwe amasunga kukhulupirika kwa kuchuluka kwa ma cell ndikuwongolera kutulutsa kwamadzimadzi panthawi yoyezera thupi.
Ma Lyse Reagents a Leukocyte Kusiyana:Njira zothanirana ndi vutoli zomwe zimasankha lyse maselo ofiira amagazi kuti apange maselo oyera amagazi kuti awonedwe.
Analyzer Cleaning Solutions:Madzi ochapira ma enzyme opangidwa kuti achotse mapuloteni ochuluka ndi zotsalira za utoto kuchokera ku mizere yamadzimadzi yamkati ndi ma cell otuluka.
Mafuta a Sheath Concentrated:Madzi a Hydrodynamic opangidwa kuti aziyika pakati pa mitsinje mkati mwa zipinda zowonera.

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