WDF Dye for Sysmex 5-Part Hematology Analyzers ndi chipangizo chapadera cha fulorosenti chomwe chimapangidwira makina opangira ma optical flow cytometry, kulowa m'mitsempha yamagazi oyera amwazi kuti amange ma nucleic acid a cell kuti awerengere ma cell ndi ma scattergram.
Kodi Hematology Stain / Dye Reagents Ndi Chiyani?
Makina opangira utoto wa hematology ndi mankhwala apadera a fluorophore opangidwa kuti azizindikira ma tchanelo mkati mwa-zowerengera zama cell angapo. Mosiyana ndi madontho opaka magazi opaka pamanja omwe amagwiritsidwa ntchito mu microscope wamba, ma reagents awa ali ndi mankhwala opangidwa ndi fulorosenti osungunuka mu zonyamulira za organic. Pa yodziwikiratu selo kukonzekera, ndi reagent akulowa lysed maselo kumanga okhudza maselo ambiri RNA ndi DNA, kupanga enieni fulorosisi chizindikiro pansi laser chisangalalo.
Udindo mu Gulu la Maselo / Kuzindikira
Zida zopangira utoto wa fluorescent zimathandizira kuzindikira-maselo am'mbali mwa kuyeza kufalikira kwa kuwala ndi mphamvu ya fulorosenti mkati mwa cell cell.
Kumanga kwa Nucleic Acid:Fluorophore imamangiriza ku RNA ndi DNA ya intracellular, imatulutsa kuwala kolingana ndi ma nucleic acid omwe ali ndi ma cell a cell ndi metabolism.
Kusiyana kwa Leukocyte:Amalekanitsa ma lymphocyte okhwima, ma monocyte, neutrophils, ndi eosinophils m'magulu osiyana -pamagulu awiri a dimensional scattergrams.
Kuyimitsa Maselo Osadziwika:Imazindikiritsa ma lymphocyte atypical ndi maselo osakhwima kutengera ma siginecha okwera a fulorosenti kuchokera ku kuchuluka kwa RNA ndi DNA.
Kuzindikira Channel
Makina osanthula a 5-part hematology analyzer amagwiritsa ntchito mayendedwe a fulorosenti kuti asinthe kuchuluka kwa ma cell:
WDF Channel:Imayesa kuchuluka kwa maselo oyera amwazi, ma atypical lymphocyte, ndi ma granulocyte osakhwima pogwiritsa ntchito mbali ya fluorescence ndi kubalalitsa mbali.
Channel ya WNR:Imasanthula kuchuluka kwa maselo oyera amwazi ndi maselo ofiira amagazi popatula ma siginecha a nyukiliya a fluorescence.
WPC Channel:Zowonetsera za ma cell precursor, ma lymphocyte osadziwika, ndi ma cell a progenitor cell.
RET Channel:Imawerengera reticulocytes ndi erythrocyte okhwima poyang'ana intracellular ribosomal RNA.
PLT Channel:Amapereka kuwerengera kwa mapulateleti owoneka bwino pogwiritsa ntchito zolembera za fulorosenti kuti apewe kusokonezedwa ndi tizidutswa ta cell kapena ma microcytic erythrocytes.
Yogwirizana Analyzer Series
Makina 5 Osanthula Hematology:Mapulaneti apamwamba{{0}panjira, malo oyimirira okha{1}}mayunitsi okhazikika, ndi ma compact benchtop system.
Zozindikiritsa Zofananira:STAC-DYE-SYSWNR, STAC-DYE-SYSWDF, STAC-DYE-SYSWPC, STAC-DYE-SYSRET, STAC-DYE-SYSPLT
Zofotokozera Zamalonda
|
Parameter |
Kufotokozera |
|
Product Model |
STAC-DYE-SYSWDF |
|
Chandamale Channel |
WDF (White Blood Cell Differential) |
|
Molekyu Yogwira |
Polymethine Fluorescent Compound |
|
Chisangalalo / Emission Wavelength |
633-635 nm / 650-670 nm |
|
Mtundu wa pH |
7.00-7.40 pa 25 digiri |
|
Zolondola (CV) |
WBC Yocheperapo kapena yofanana ndi 3.0%, NEUT% Ochepa kapena ofanana ndi 5.0%, LYMPH% Ochepa kapena ofanana ndi 5.0% |
|
Kugwirizana ($R^2$) |
Kuposa kapena kufanana ndi 0.99 |
|
Alumali Moyo / Open Vial Kukhazikika |
Miyezi ya 18 (Yosatsegulidwa) / Masiku 60 (Yotsegulidwa) pa digiri ya 2 -35 digiri |
Ntchito & Reagent Ntchito
Kusiyana kwa 5-Part Leukocyte:Madontho a intracellular nucleic acids kuti alekanitse ma lymphocyte, monocytes, neutrophils, ndi eosinophils kukhala masango a scattergram.
Kuchuluka kwa Granulocyte:Amapanga ma siginecha amphamvu a fulorosenti kwa ma cell oyambira kuti athe kuwerengera mokhazikika popanda kuwunika kwapamanja kwa smear.
Kuzindikira kwa Atypical Lymphocyte:Imazindikiritsa milingo yokwezeka ya RNA mu ma leukocyte oyambilira kuti ayambitse zidziwitso zowunikira zokha.
Phukusi / Kusungirako
Kutentha Kosungirako:2 -35 digiri. Sungani muzotengera zoyambirira zamdima kutali ndi kuwala kwachindunji.
Zofotokozera za Chotengera:-Mabotolo amtundu wa polyethylene amber ochuluka kwambiri okhala ndi induction{1}}otsekedwa.
Zoyendera:Zovomerezeka zonyamula katundu mozungulira. Tetezani kuzizindikiro zosachepera madigiri 0.
Zogwirizana ndi Hematology Reagents
STAC-DYE-SYSWNR:Fluorescent dye reagent yowerengera kuchuluka kwa maselo oyera amwazi komanso kusanthula kwa maselo ofiira amagazi.
STAC-DYE-SYSWPC:Mkulu-mwanjira inayake ya fulorosenti ya madontho a fulorosenti yowunikira ma cell ndi ma leukocyte.
STAC-DYE-SYSRET:Fluorescent nucleic acid banga la reticulocyte magawo a quantification.
STAC-DYE-SYSPLT:Optical fluorescent dye reagent kuti mudziwe bwino za mapulateleti mu zitsanzo zovuta.
FAQ
Q: Kodi utoto wa WDF umalepheretsa bwanji kulumikizana pakati pa neutrophils ndi eosinophils pa scattergrams?
A: Mapangidwewa amagwira ntchito limodzi ndi ma lyse reagents omwe amasankha ma cell membranes. Utotowo umalumikizana mu nyukiliya ya DNA ndi cytoplasmic RNA, kutulutsa ma fluorescence ammbali ndi kufalikira kwam'mbali kwa cell iliyonse.
Q: Kodi kukhazikika kwa zotengera zotsegula zotsegula ndi ziti?
A: Zotengera zotseguka zimakhalabe zokhazikika kwa masiku 60 zikasungidwa pakati pa 2 digiri ndi 35 digiri. The zosungunulira chiphunzitso kupewa yogwira pawiri kuwonongeka ndi zosungunulira evaporation pansi muyezo zasayansi chinyezi zinthu.
Q: Kodi ma reagents a utotowa angayikidwe mu mizere ya fluidic popanda kusintha kwa fluidic?
A: Inde. The reagent imakhala yofananira kukhuthala kwa fluidic, mbiri ya laser mayamwidwe, ndi magawo ogwirira ntchito, kulola kulumikizana mwachindunji ndi njira zofananira.
Q: Kodi batch-ku{1}}kukhazikika kwa fulorosenti imawunikidwa bwanji panthawi yopanga?
A: Liquid chromatography amawongolera amatsimikizira kuyera kwa mamolekyu pagulu lililonse lopanga, kutsatiridwa ndi kuyesa-makanema angapo kuti asunge ma scattergram cluster positioning posmobility mkati Ochepera kapena ofanana ndi 2.0%.
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